不結(jié)球白菜生長(zhǎng)發(fā)育過程中表型變化AgriPheno提供農(nóng)作物重要性狀功能基因定位服務(wù)，結(jié)合芯片和高通量測(cè)序開發(fā)材料間的SNP位點(diǎn)，并結(jié)合高通量表型測(cè)試，提供基因型-表型-育種的整套實(shí)驗(yàn)流程設(shè)計(jì)與服務(wù)。
超高通量基因分型-分子標(biāo)記檢測(cè)平臺(tái)-Nexar
Nexar系統(tǒng)是快速、自動(dòng)化的內(nèi)聯(lián)儀器，包括Nexar?模塊化內(nèi)聯(lián)液處理與分析系統(tǒng)、Soellex?高通量PCR水浴熱循環(huán)系統(tǒng)和Araya?內(nèi)聯(lián)熒光檢測(cè)系統(tǒng)，可支持樣本和陣列的高通量處理。Nexar利用創(chuàng)新的ArrayTapeTM（陣列卷帶）系列耗材，能夠在高精確度和準(zhǔn)確性下運(yùn)行。同時(shí)配備KASP Array Tape Master mix進(jìn)行競(jìng)爭(zhēng)性等位基因特異性PCR，可在廣泛的基因組DNA樣品中，對(duì)SNPs和特定位點(diǎn)上的InDels進(jìn)行精準(zhǔn)的雙等位基因分型。
Nexar系統(tǒng)模塊化內(nèi)聯(lián)平臺(tái)與Array Tape的設(shè)計(jì)實(shí)現(xiàn)了基于微孔板技術(shù)的多功能性，并且?guī)缀跸耸謩?dòng)操作和復(fù)雜的儀器操作。這種靈活的微孔板替代品可促進(jìn)整個(gè)實(shí)驗(yàn)室的內(nèi)聯(lián)和整合自動(dòng)化，并有助于終點(diǎn)PCR，qPCR和終點(diǎn)等溫DNA擴(kuò)增的應(yīng)用。每個(gè)96孔，384孔和768孔陣列都標(biāo)有獨(dú)特的條形碼標(biāo)簽，確保在處理過程中和加工后準(zhǔn)確識(shí)別各個(gè)樣品。此外，還可提供定制化的陣列，包括RNAse，DNAse和無熱原選項(xiàng)。
Nexar 系統(tǒng)
Nexar? |
Soellex? |
Araya? |
? Nexar?
Nexar作為超高通量解決方案，是一套用于Array Tape（陣列卷帶）樣品和試劑處理的內(nèi)聯(lián)液體處理系統(tǒng)。該儀器提供分裝、密封和源板存儲(chǔ)，以及可選進(jìn)程，如孵育和脫水。這一靈活的模塊配置可以處理各種應(yīng)用進(jìn)程。
? Soellex?
Soellex為三室水浴熱循環(huán)系統(tǒng)，能夠在一輪運(yùn)行中同時(shí)熱循環(huán)多達(dá)三個(gè)Array Tape（陣列卷帶）線軸（230,400個(gè)反應(yīng)孔）或152個(gè)微孔板（384孔）。
? Araya?
Araya是內(nèi)聯(lián)熒光檢測(cè)系統(tǒng)，專為Array Tape（陣列卷帶）的自動(dòng)掃描而設(shè)計(jì)。該系統(tǒng)可作為獨(dú)立儀器或Nexar的內(nèi)聯(lián)模塊使用。
? Intellics?
創(chuàng)新的配套軟件提供集中數(shù)據(jù)管理、儀器監(jiān)控、智能運(yùn)行優(yōu)化、Protocol生成和簡(jiǎn)化數(shù)據(jù)分析。
應(yīng)用領(lǐng)域
? 糧食作物：水稻、小麥、玉米、土豆等
? 模式植物：擬南芥、煙草、二穗短柄草等
? 蔬菜作物：生菜、番茄、甜菜、黃瓜、甘藍(lán)、菠菜等
? 模式動(dòng)物：人、小白鼠、斑馬魚等
? 常見牲畜：牛、豬、山羊等
應(yīng)用案例
圖1 典型的基因分型富集圖
注：每個(gè)數(shù)據(jù)點(diǎn)代表一個(gè)獨(dú)立的DNA樣本的熒光信號(hào)，相同基因型的樣本會(huì)發(fā)出相似水平的熒光，因此富集在一起。
服務(wù)項(xiàng)目
? 目標(biāo)基因/性狀標(biāo)記開發(fā) ? 全基因組標(biāo)記開發(fā) ? 遺傳圖譜的構(gòu)建 ? 圖位克隆 ? 背景篩查 ? 蔬菜等純度檢測(cè) ? 植物身份鑒定/分群 |  |
服務(wù)特點(diǎn)
? 適用于超高通量自動(dòng)化檢測(cè)，液體處理、孵育與檢測(cè)等流程一體化
? 反應(yīng)體系微型化，顯著降低單個(gè)數(shù)據(jù)點(diǎn)的成本
? 兼容粗提的DNA樣本，并可靈活選用化學(xué)試劑
? 專業(yè)化生物信息平臺(tái)及團(tuán)隊(duì)
? 大規(guī)模數(shù)據(jù)存儲(chǔ)及數(shù)據(jù)處理服務(wù)器
客戶提供
? 基因或指定區(qū)間信息
? 符合要求的供體/受體材料的DNA、葉片或種子等植物組織
服務(wù)周期
? 根據(jù)特定序列中變異位點(diǎn)設(shè)計(jì)標(biāo)記：10-25個(gè)工作日
? 根據(jù)特定供體與特定回交親本開發(fā)：10-20個(gè)工作日
? 基因初步定位：30～40個(gè)工作日
? 基因精細(xì)定位：30～40個(gè)工作日
? 已克隆基因序列開發(fā): 15-30個(gè)工作日
? 全基因組標(biāo)記開發(fā)：4-6個(gè)月
? 未克隆已定位基因標(biāo)記開發(fā)：根據(jù)性狀復(fù)雜程度確定
視頻鏈接：https://v.youku.com/v_show/id_XNDAzMDE5NjU2OA==.html?spm=a2h3j.8428770.3416059.1
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